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#microscopy
6 posts4 participants1 post today
A modular hand-held #OpenSource #fluorescent #camera unit for #wound #imaging:
-custom #3Dprinted housing
-based on #RaspberryPi & #ArduCam 21MP
-3 independent #LED channels
https://doi.org/10.1038/s42003-025-08423-y
#DIYbio #lab #instruments #fluorescence #microscope #microscopy #NO #pH #ML
A versatile #OpenSource one- & two-photon light-sheet #microscope (#LSM) design:
Preprint: https://doi.org/10.1101/2023.07.10.548107
GitHub: https://github.com/LJPZebra/OLU
It's a bit magical how adding diversity to a training dataset improves the result of the model. I was analyzing one microscopy experiment, and the segmentation model, trained on the same experiment, was doing well on this particular movie but not on others. I added a few more experiments to the training set, and now the model does much better even on experiments outside of the training set.
It's Day 2 of the Warwick-Vanderbilt Mechanobiology Meeting.
Speakers this morning:
Mohan Balasubramanian (Warwick)
Marija Zanic (Vanderbilt)
Nigel Burroughs (Warwick)
Andrea Page-McCaw (Vanderbilt)
Michael Smutny (Warwick)
Vos Bart (Goettingen)
Karuna Sampath (Warwick)
Early-career researchers, apply for a funded place at our Workshop 'Macro to Micro: Quantitative Plant Imaging Across Scales'.
https://www.biologists.com/workshops/february-2026-2/
Application deadline: Friday 15 August 2025
#BrightEyesTTM: #OpenSource #FPGA-based multi-channel time-tagging module (#TTM) for democratising single-photon (SP) #microscopy:
-parallel multiple #SP event tagging precision: 30 ps
-multiple synchronisation event precision: 4 ns
-requires #LabVIEW
-cost ~$3000
Article: https://doi.org/10.1038/s41467-022-35064-0
Web: https://brighteyes-ttm.readthedocs.io/en/latest/
GitHub: https://github.com/VicidominiLab/BrightEyes-TTM
#DIYbio #lab #instruments #LSM #FLISM #FLFS #fluorescence #spectroscopy #imaging #Python
Meet the Nikon Microscope Collector - Uli Koch | Teaser | 100 Years of Nikon Microscopy
https://www.youtube.com/watch?v=BT9WnKszSsM #Microscopy @plantscience
Following on from their successful meeting in 2023, @J_Cell_Sci are delighted to announce a second iteration of the Imaging Cell Dynamics Meeting to be held in 2026.
Find out more:
#MSLASpheroidStamp: #OpenSource 3D #cell #spheroids for everyone:
-#3Dprinted molds/stamps
-time-lapse #confocal #microscopy of spheroids
-#cytotoxicity screens
Preprint: https://doi.org/10.1101/2024.05.12.593682
GitHub: https://github.com/arteys/MSLASpheroidStamp
#DIYbio #lab #instruments #MSLA #3Dprinting #bioprinting #imaging
ѦਹѤʞ 
Continued thread
...now published in Nature Communications:
https://doi.org/10.1038/s41467-024-49131-1
#OpenSource #DIYbio #lab #instruments #imaging #microscopy
Continued thread
...now published in Nature Communications:
https://doi.org/10.1038/s41467-024-46770-2
#OpenSource #DIYbio #lab #instruments #imaging #microscopy
#Vegetative #electron #microscopy” is #nonsense. And yet several scientific #papers have included the phrase over the past few years.
When two papers from the 1950s were digitized, two columns of text may have been read together incorrectly, the “vegetative” from one column smushed up with the “electron microscopy” in the other.
The #researchers discovered the phrase started showing up in responses generated by #OpenAI’s GPT 3.0, which was released in 2020.
https://www.freethink.com/artificial-intelligence/vegetative-electron-microscopy
FreethinkAI thinks “vegetative electron microscopy” is real — it’s notThe appearance of a nonsense term in multiple scientific papers has exposed how flawed data in training sets can become embedded in AIs.
And the #nakeddiefriday comes back with a bang. Today's guest is a Philips product, p/n PCB83C851. This is a 8051 derivative, of which Philips made quite a few.
I'll do a short tour thread with a small bonus at the end.
SiPron page:https://siliconpr0n.org/archive/doku.php?id=infosecdj:philips:pcb83c851
We had some code for image randomisation which ran very slowly in ImageJ.
Pro: easy to use by labbies
Con: very slow, like really slowww
Decided to rewrite the whole thing in python last night. In testing this morning, it seems to be 100 times faster! It uses a different approach so the comparison is not entirely fair, but still #GoMe
I just love the way these little guys move like look at him goooooo 
excuse the low quality my lenses are dusty and I was feeling lazy last night
Do you have a microscopy image you would like to share? Add it to the FocalPlane image gallery.
#Altair-LSFM: A high-res, easy-to-build light-sheet #OpenSource #microscope for sub-cellular #imaging:
-lateral/axial res: ~235/350 nm across 266-µ FOV after deconvolution
Preprint: https://doi.org/10.1101/2025.04.08.647739
Repo: https://thedeanlab.github.io/altair/
#DIYbio #lab #instruments #microscopy #LSM #fluorescence
