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#fluorescencefriday

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Daniel MacPhee 🔬🧬🧫🇨🇦<p>Had to revisit our paper from 2018 for a future project…boy I love the immunofluorescence microscopy we did on the human placenta. <br><a href="https://mas.to/tags/FluorescenceFriday" class="mention hashtag" rel="nofollow noopener" target="_blank">#<span>FluorescenceFriday</span></a> <a href="https://mas.to/tags/Science" class="mention hashtag" rel="nofollow noopener" target="_blank">#<span>Science</span></a> <a href="https://mas.to/tags/Placenta" class="mention hashtag" rel="nofollow noopener" target="_blank">#<span>Placenta</span></a> <a href="https://mas.to/tags/ReproSci" class="mention hashtag" rel="nofollow noopener" target="_blank">#<span>ReproSci</span></a> <br><a href="https://bmcdevbiol.biomedcentral.com/articles/10.1186/s12861-018-0178-0" rel="nofollow noopener" translate="no" target="_blank"><span class="invisible">https://</span><span class="ellipsis">bmcdevbiol.biomedcentral.com/a</span><span class="invisible">rticles/10.1186/s12861-018-0178-0</span></a></p>
N-Aryl Felinine 🌲<p>terbium something (idk?) <a href="https://mastodon.social/tags/fluorescencefriday" class="mention hashtag" rel="nofollow noopener" target="_blank">#<span>fluorescencefriday</span></a></p>
Ernesto<p>Linda semana, sumergido en el mundo microscópico…<br>🔬🦠</p><p><a href="https://mastodon.uy/tags/mitochondria" class="mention hashtag" rel="nofollow noopener" target="_blank">#<span>mitochondria</span></a><br><a href="https://mastodon.uy/tags/fluorescencefriday" class="mention hashtag" rel="nofollow noopener" target="_blank">#<span>fluorescencefriday</span></a></p>
Eishi Noguchi<p><a href="https://masto.ai/tags/FluorescenceFriday" class="mention hashtag" rel="nofollow noopener" target="_blank">#<span>FluorescenceFriday</span></a></p><p>Front yard😊<br>Not really fluorescence, but like fluorescence 😉</p><p><a href="https://masto.ai/tags/Flower" class="mention hashtag" rel="nofollow noopener" target="_blank">#<span>Flower</span></a> <a href="https://masto.ai/tags/Fluorescence" class="mention hashtag" rel="nofollow noopener" target="_blank">#<span>Fluorescence</span></a> <a href="https://masto.ai/tags/Plant" class="mention hashtag" rel="nofollow noopener" target="_blank">#<span>Plant</span></a> <a href="https://masto.ai/tags/Yard" class="mention hashtag" rel="nofollow noopener" target="_blank">#<span>Yard</span></a> <a href="https://masto.ai/tags/Photography" class="mention hashtag" rel="nofollow noopener" target="_blank">#<span>Photography</span></a></p>
Eishi Noguchi<p><a href="https://masto.ai/tags/FluorescenceFriday" class="mention hashtag" rel="nofollow noopener" target="_blank">#<span>FluorescenceFriday</span></a> </p><p>Some nice colors of Dragon fruit 😊😋<br>This reminds me that I should plan some fluorescence microscopy experiments 🤔</p><p><a href="https://masto.ai/tags/DragonFruit" class="mention hashtag" rel="nofollow noopener" target="_blank">#<span>DragonFruit</span></a> <a href="https://masto.ai/tags/Fruits" class="mention hashtag" rel="nofollow noopener" target="_blank">#<span>Fruits</span></a> <a href="https://masto.ai/tags/Photography" class="mention hashtag" rel="nofollow noopener" target="_blank">#<span>Photography</span></a> <a href="https://masto.ai/tags/Fluorescence" class="mention hashtag" rel="nofollow noopener" target="_blank">#<span>Fluorescence</span></a> <a href="https://masto.ai/tags/Science" class="mention hashtag" rel="nofollow noopener" target="_blank">#<span>Science</span></a> <a href="https://masto.ai/tags/Experiment" class="mention hashtag" rel="nofollow noopener" target="_blank">#<span>Experiment</span></a></p>
Moritz Negwer<p>New publication from Kwanghun Chung lab at MIT improves on their SWITCH approach and eFLASH stochastic electrotransport to improve antibody labeling by gradually shifting the microenvironment. Looks interesting, I wonder how well it can be adapted to other labeling protocols.</p><p>Uniform volumetric single-cell processing for organ-scale molecular phenotyping<br>Yun et al., Nature Biotechnology 2025<br><a href="https://doi.org/10.1038/s41587-024-02533-4" rel="nofollow noopener" translate="no" target="_blank"><span class="invisible">https://</span><span class="ellipsis">doi.org/10.1038/s41587-024-025</span><span class="invisible">33-4</span></a></p><p><a href="https://mstdn.science/tags/neuroscience" class="mention hashtag" rel="nofollow noopener" target="_blank">#<span>neuroscience</span></a> <a href="https://mstdn.science/tags/tissueclearing" class="mention hashtag" rel="nofollow noopener" target="_blank">#<span>tissueclearing</span></a> <a href="https://mstdn.science/tags/lightsheet" class="mention hashtag" rel="nofollow noopener" target="_blank">#<span>lightsheet</span></a> <a href="https://mstdn.science/tags/microscopy" class="mention hashtag" rel="nofollow noopener" target="_blank">#<span>microscopy</span></a> <a href="https://mstdn.science/tags/fluorescenceFriday" class="mention hashtag" rel="nofollow noopener" target="_blank">#<span>fluorescenceFriday</span></a></p>
Mick Craig<p>I missed <a href="https://fediscience.org/tags/FluorescenceFriday" class="mention hashtag" rel="nofollow noopener" target="_blank">#<span>FluorescenceFriday</span></a> but here's an image for one from the manuscripts on the nearly-finished pile. We've found a genetic marker for the deep CA1 pyramidal cells. Magenta is calbindin (IHC), cyan is the other cell type (AAV-TdTom). Less than 5% overlap. Coming to BioRxiv by Easter, hopefully! <a href="https://fediscience.org/tags/Neuroscience" class="mention hashtag" rel="nofollow noopener" target="_blank">#<span>Neuroscience</span></a></p>
Dr. Heather Etchevers<p>You can check it out here: <a href="https://doi.org/10.1016/j.jid.2024.10.595" rel="nofollow noopener" translate="no" target="_blank"><span class="invisible">https://</span><span class="ellipsis">doi.org/10.1016/j.jid.2024.10.</span><span class="invisible">595</span></a></p><p><a href="https://mas.to/tags/fluorescencefriday" class="mention hashtag" rel="nofollow noopener" target="_blank">#<span>fluorescencefriday</span></a> every day...</p><p>We are especially grateful to CMN patient advocacy groups, MELCAYA, AFM-Téléthon, and the Institut Marseille Maladies Rares (MarMaRa), for their support.</p><p>Bon bout d'an à tous ! (Happy nearly-New Year to all!)</p><p>-fin-</p>
Teixi<p><span class="h-card" translate="no"><a href="https://synapse.cafe/@MFM_BIC" class="u-url mention" rel="nofollow noopener" target="_blank">@<span>MFM_BIC@synapse.cafe</span></a></span> <span class="h-card" translate="no"><a href="https://mastodon.social/@MFM_BIC" class="u-url mention" rel="nofollow noopener" target="_blank">@<span>MFM_BIC</span></a></span> </p><p>ICYMI you can follow tags in here:</p><p><a href="https://mastodon.social/tags/FluorescenceFriday" class="mention hashtag" rel="nofollow noopener" target="_blank">#<span>FluorescenceFriday</span></a> <br><a href="https://mastodon.social/tags/NeuroImaging" class="mention hashtag" rel="nofollow noopener" target="_blank">#<span>NeuroImaging</span></a> <br><a href="https://mastodon.social/tags/CellBiology" class="mention hashtag" rel="nofollow noopener" target="_blank">#<span>CellBiology</span></a></p>
FocalPlane<p>On <a href="https://biologists.social/tags/FluorescenceFriday" class="mention hashtag" rel="nofollow noopener" target="_blank">#<span>FluorescenceFriday</span></a>, we’re highlighting this beautiful SIM image from <span class="h-card" translate="no"><a href="https://mas.to/@dgaboriau" class="u-url mention" rel="nofollow noopener" target="_blank">@<span>dgaboriau</span></a></span> These human fibroblasts are labelled to visualise mitochondria (Tom20) in yellow, alpha-tubulin in pink and actin in grey.<br><a href="https://focalplane.biologists.com/2024/10/11/featured-image-with-david-gaboriau/" rel="nofollow noopener" translate="no" target="_blank"><span class="invisible">https://</span><span class="ellipsis">focalplane.biologists.com/2024</span><span class="invisible">/10/11/featured-image-with-david-gaboriau/</span></a></p>
Eishi Noguchi<p><a href="https://masto.ai/tags/FluorescenceFriday" class="mention hashtag" rel="nofollow noopener" target="_blank">#<span>FluorescenceFriday</span></a></p><p>Some nice telomere signals, I think experiments are working </p><p><a href="https://masto.ai/tags/Telomere" class="mention hashtag" rel="nofollow noopener" target="_blank">#<span>Telomere</span></a> <a href="https://masto.ai/tags/Fluorescence" class="mention hashtag" rel="nofollow noopener" target="_blank">#<span>Fluorescence</span></a> <a href="https://masto.ai/tags/CellBiology" class="mention hashtag" rel="nofollow noopener" target="_blank">#<span>CellBiology</span></a> <a href="https://masto.ai/tags/MolecularBiology" class="mention hashtag" rel="nofollow noopener" target="_blank">#<span>MolecularBiology</span></a> <a href="https://masto.ai/tags/GraduateSchool" class="mention hashtag" rel="nofollow noopener" target="_blank">#<span>GraduateSchool</span></a></p>
Carsen Stringer (she/her)<p>for <a href="https://neuromatch.social/tags/FluorescenceFriday" class="mention hashtag" rel="nofollow noopener" target="_blank">#<span>FluorescenceFriday</span></a>, here's a video of two-photon imaging 🔬 from mouse visual cortical neurons at 0.5x speed in response to many natural images <a href="https://neuromatch.social/tags/neuroscience" class="mention hashtag" rel="nofollow noopener" target="_blank">#<span>neuroscience</span></a> (paper here: <a href="https://www.biorxiv.org/content/10.1101/2024.06.30.601394v1" rel="nofollow noopener" translate="no" target="_blank"><span class="invisible">https://www.</span><span class="ellipsis">biorxiv.org/content/10.1101/20</span><span class="invisible">24.06.30.601394v1</span></a>)</p>
Kristine Willis<p>Happy <a href="https://mstdn.science/tags/fluorescenceFriday" class="mention hashtag" rel="nofollow noopener" target="_blank">#<span>fluorescenceFriday</span></a>, let’s see those gorgeous images! </p><p><a href="https://mstdn.science/tags/FluorescenceFriday" class="mention hashtag" rel="nofollow noopener" target="_blank">#<span>FluorescenceFriday</span></a></p>
FocalPlane<p>It’s <a href="https://biologists.social/tags/FluorescenceFriday" class="mention hashtag" rel="nofollow noopener" target="_blank">#<span>FluorescenceFriday</span></a> &amp; we’re highlighting Shivangi Verma’s (@shivangiv10) image of a <a href="https://biologists.social/tags/zebrafish" class="mention hashtag" rel="nofollow noopener" target="_blank">#<span>zebrafish</span></a> larval brain. The larva expressing GCaMP6f was embedded in agarose &amp; imaged using a Zeiss Lightsheet microscope. Find out more about Shivangi's research: <br><a href="https://focalplane.biologists.com/2024/08/30/featured-image-with-shivangi-verma/" rel="nofollow noopener" translate="no" target="_blank"><span class="invisible">https://</span><span class="ellipsis">focalplane.biologists.com/2024</span><span class="invisible">/08/30/featured-image-with-shivangi-verma/</span></a></p>
Falk Schneider<p>Happy <a href="https://mas.to/tags/FluorescenceFriday" class="mention hashtag" rel="nofollow noopener" target="_blank">#<span>FluorescenceFriday</span></a> </p><p>Some actin action always - amazes me (and gets me safely into the weekend) 😜 </p><p>Timelapse imaging on Zeiss880 of HEK cells expressing Lifeact-mEGFP<br>LUT is Cyan Hot (quite like that one)</p>
Falk Schneider<p><a href="https://mas.to/tags/FluorescenceFriday" class="mention hashtag" rel="nofollow noopener" target="_blank">#<span>FluorescenceFriday</span></a><br>(is this a thing on <a href="https://mas.to/tags/Mastodon" class="mention hashtag" rel="nofollow noopener" target="_blank">#<span>Mastodon</span></a> - if not let's make it one!)</p><p>Nuclei labelled with EGFP-H2B.<br>MIP of a small z-stack at two frames per minute.</p><p>Loads of action, movement and cell divisions, in the developing zebra fish hindbrain 🐟🧠</p>
Eishi Noguchi<p>It’s been a busy day, but I had the pleasure of meeting a new group of students in the graduate programs 😊</p><p>It already feels like Friday to me 🤔 While it’s not Friday here in Pennsylvania just yet, it is in many countries, including where some of our students are from 🌍</p><p>So, <a href="https://masto.ai/tags/FluorescenceFriday" class="mention hashtag" rel="nofollow noopener" target="_blank">#<span>FluorescenceFriday</span></a> </p><p><a href="https://masto.ai/tags/Orientation" class="mention hashtag" rel="nofollow noopener" target="_blank">#<span>Orientation</span></a> <a href="https://masto.ai/tags/GraduteSchool" class="mention hashtag" rel="nofollow noopener" target="_blank">#<span>GraduteSchool</span></a> <a href="https://masto.ai/tags/BiomedicalScience" class="mention hashtag" rel="nofollow noopener" target="_blank">#<span>BiomedicalScience</span></a> <a href="https://masto.ai/tags/GraduateStudents" class="mention hashtag" rel="nofollow noopener" target="_blank">#<span>GraduateStudents</span></a></p>
FocalPlane<p>It’s time for <a href="https://biologists.social/tags/FluorescenceFriday" class="mention hashtag" rel="nofollow noopener" target="_blank">#<span>FluorescenceFriday</span></a>! Today, in our <a href="https://biologists.social/tags/FeaturedImage" class="mention hashtag" rel="nofollow noopener" target="_blank">#<span>FeaturedImage</span></a> post we highlight the research of Upasana Gupta who acquired this beautiful image of a zebrafish larva eye.</p><p><a href="https://focalplane.biologists.com/2024/08/02/featured-image-with-upasana-gupta/" rel="nofollow noopener" translate="no" target="_blank"><span class="invisible">https://</span><span class="ellipsis">focalplane.biologists.com/2024</span><span class="invisible">/08/02/featured-image-with-upasana-gupta/</span></a></p>
Eishi Noguchi<p><a href="https://masto.ai/tags/FluorescenceFriday" class="mention hashtag" rel="nofollow noopener" target="_blank">#<span>FluorescenceFriday</span></a></p><p>Some proliferating, some not 🤔 <br>My wife was doing some cell proliferation assays 😊 </p><p>Photo by Chiaki</p>
FocalPlane<p>On this <a href="https://biologists.social/tags/FluorescenceFriday" class="mention hashtag" rel="nofollow noopener" target="_blank">#<span>FluorescenceFriday</span></a>, our <a href="https://biologists.social/tags/FeaturedImage" class="mention hashtag" rel="nofollow noopener" target="_blank">#<span>FeaturedImage</span></a> shows a Drosophila embryo undergoing syncytial nuclear division from Jovan Brockett. Read more about Jovan’s research in our post: <a href="https://focalplane.biologists.com/2024/07/19/featured-image-with-jovan-brockett/" rel="nofollow noopener" translate="no" target="_blank"><span class="invisible">https://</span><span class="ellipsis">focalplane.biologists.com/2024</span><span class="invisible">/07/19/featured-image-with-jovan-brockett/</span></a></p>
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