Does anyone who runs automated column chromatography ever turn up the gain on the UV-vis detector? I've never done it but I have some things with unusually low absorbance for their structures and I've been tempted to, but don't know if it would be helpful or by how much I should try (the options are 1x - 10x on the combiflash).
(also my poor little fluorophore chemist brain is fried by the idea of absorbance having a gain option...)